Study of Placental Lactogen Gene Polymorphism and Its Association With Milk Production Traits in The Holstein Cows

Authors

  • Arash Omidi Department of Animal Health Management, shiraz university, PO. Box 71345-1731 ,shiraz, Phars Province, I.R. Iran
  • Homayoun Farhangfar Department of Animal Science, Birjand University, PO. Box 331, Birjand, South Khorasan Province, I.R. Iran
  • Mohammad Bagher Montazer Torbati Department of Animal Science, Birjand University, PO. Box 331, Birjand, South Khorasan Province, I.R. Iran
  • Mohammad Mahmoudzadeh Department of Animal Science, Birjand University, PO. Box 331, Birjand, South Khorasan Province, I.R. Iran
Abstract:

Placental Lactogen is a polypeptide hormone that is produced by the Placenta, also known as chorionic somatomammotropin hormone. It has both Growth Hormone and Prolactin activities on growth, lactation, and luteal steroid production. The objective of this study was to investigate the bovine Placental Lactogen (bPL) gene polymorphism of Holstein cows in Razavi Khorasan province. Blood samples were collected from 150 dairy cattle from six herds. DNA extraction was performed by salting out method. A fragment of 449 bp from intron 1 was amplified by the polymerase chain reaction and analyzed by single-strand conformation polymorphism to get the patterns of single-stranded DNA separated by native polyacrylamide gel electrophoresis and visualized by silver staining. Six genotypes were revealed with the frequencies of 0.283 (AA), 0.085 (AB), 0.292 (AC), 0.019 (CC), 0.292 (AD) and 0.029 (DD). The allele frequencies for A, B, C and D were 0.6179, 0.0425, 0.1651 and 0.1745, respectively.Chi-square test didn't confirm Hardy-Weinberg (H-W) equilibrium for this locus. Associations between polymorphisms and the traits studied were evaluated using the MIXED procedure of the SAS 9.1 software. Results showed that the polymorphism of the bPL gene is significantly is associated with fat percent (P=0.012)

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

study of placental lactogen gene polymorphism and its association with milk production traits in the holstein cows

placental lactogen is a polypeptide hormone that is produced by the placenta, also known as chorionic somatomammotropin hormone. it has both growth hormone and prolactin activities on growth, lactation, and luteal steroid production. the objective of this study was to investigate the bovine placental lactogen (bpl) gene polymorphism of holstein cows in razavi khorasan province. blood samples we...

full text

the study of aaag repeat polymorphism in promoter of errg gene and its association with the risk of breast cancer in isfahan region

چکیده: سرطان پستان دومین عامل مرگ مرتبط با سرطان در خانم ها است. از آنجا که سرطان پستان یک تومور وابسته به هورمون است، می تواند توسط وضعیت هورمون های استروئیدی شامل استروژن و پروژسترون تنظیم شود. استروژن نقش مهمی در توسعه و پیشرفت سرطان پستان ایفا می کند و تاثیر خود را روی بیان ژن های هدف از طریق گیرنده های استروژن اعمال می کند. اما گروه دیگری از گیرنده های هسته ای به نام گیرنده های مرتبط به ا...

15 صفحه اول

Single nucleotide polymorphism of the lactoferrin gene and its association with milk production and reproduction traits in Iranian Holstein cattle

Bovine lactoferrin (LTF) is a member of the transferrin family of iron-binding proteins. This protein is present in a wide variety of biological fluids and shows important physiological functions in body. In this study, 404 blood samples were collected from Holstein dairy farms in Iran. A 301 bp fragment of intron 6 in bovine LTF gene was amplified and the animals were genotyped using polymeras...

full text

Polymorphism identification, RH mapping and association of placental lactogen gene with milk production traits of dairy cows.

Bovine placental lactogen (bPL) is structurally related to prolactin (PRL) and growth hormone (GH). In synergism with steroid and thyroid hormones, bPL is crucial in stimulating the development of the mammary gland, mammary cell differentiation and function. To further explore whether bPL gene is associated with milk production traits, we herein analyzed single-nucleotide polymorphisms (SNPs) w...

full text

Single Nucleotide Polymorphism in the Promoter Region of Bovine Interleukin 8 Gene and its Association with Milk Production Traits and Somatic Cell Score of Holstein Cattle in Iran

Background: Interleukie-8 is a proinflammatory cytokine and a potent chemotactic and activation factor for neutrophils. It has been reported to be expressed in high levels by various cell types after immune stimulation and mediates neutrophil recruitment in host. Objectives: The aim of this study was to analysis of the relationship between single nucleotide polymorphisms (SNPs) at position -18...

full text

My Resources

Save resource for easier access later

Save to my library Already added to my library

{@ msg_add @}


Journal title

volume 2  issue 3

pages  650- 658

publication date 2014-03-01

By following a journal you will be notified via email when a new issue of this journal is published.

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023